Cells: Saccharomyces cerevisiae DBY8724 
Culture: Grown in synthetic minimal medium (YMD) containing 0.7% yeast
nitrogen base without amino acids and ammonium sulfate (DIFCO Bacto), 2%
glucose, 5g/l ammonium sulfate and uracil (20 mg/L).  until ~0.6-0.8 abs (OD
600) 
Varible: Analysing protein profiles in early log phase